http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109897902-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6888 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6804 |
| filingDate | 2019-04-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-11-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-11-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109897902-B |
| titleOfInvention | Rapid detection test method and kit for donkey-derived components in food |
| abstract | The invention provides a rapid detection kit for donkey-derived ingredients in food, and relates to the technical field of biological species identification. According to the invention, firstly, a donkey-derived universal internal standard gene LOC106825524 is screened, the nucleotide sequence of the donkey-derived universal internal standard gene is shown as SEQ ID NO.1, the copy number of the donkey-derived universal internal standard gene is constant in donkey species, allelic variation does not exist, and the donkey-derived universal internal standard gene can be used as a target gene for identifying donkey sources. An LAMP amplification primer is designed by taking the gene as a target sequence, and is subjected to constant-temperature amplification together with LAMP reaction liquid, and an LAMP reaction product is used for detecting the colloidal gold nucleic acid test strip. The LAMP reaction is combined with the colloidal gold nucleic acid test strip for detection to form a rapid detection kit, so that the donkey-derived component in food can be rapidly and sensitively detected, and the detection sensitivity can reach 0.2% (w/w). The kit has the advantages of simple use method, low cost, easy observation of reaction results and good specificity, and is very suitable for field real-time detection. |
| priorityDate | 2019-04-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 60.