http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109897889-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_304f3a2ba4700f3024d97500a9d6d608 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6844 |
| filingDate | 2019-04-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_852d5b48709cf26af192fa8c74fd6599 |
| publicationDate | 2019-06-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109897889-A |
| titleOfInvention | A Visual Detection Method for LAMP (Loop-Mediated Isothermal Amplification) Products |
| abstract | The invention discloses a method for visual detection of LAMP (Loop Mediated Isothermal Amplification) products, comprising the following steps: before the LAMP reaction, adding a visible light dye with a final concentration of 0.4-1 mM to the amplification system, and before the reaction starts, adding visible light dye to the amplification system The dye can be combined with the magnesium ions in the reaction system to form a light blue complex, heated in a water bath, the temperature of the reaction system is controlled at 50-70°C, and the reaction time is 15-60min. With the progress of the reaction, there will be by-product coke. Phosphate is generated, and pyrophosphate will combine with magnesium ions. After the light blue complex loses magnesium ions, it is a dark blue visible light dye. By observing the color change of the complex composed of the visible light dye and the substances in the reaction system, it can be Judge the result of the reaction. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113416799-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113416799-B |
| priorityDate | 2019-04-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 357.