http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109880885-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d40285e27f4ed83b8728b0d477404958 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-527 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 |
| filingDate | 2019-01-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e97368027659ded560244822190c032c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3122f21a425925cf968aabe2394fc6c4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_80e06bfa25deb94398f91c3d41090229 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cab5d53d8bd23b24cff2ceab4562a693 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_459ebba4e41de5b12b99646cb6bd209c |
| publicationDate | 2019-06-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109880885-A |
| titleOfInvention | A method for double fluorescence screening of β-alanine synthase |
| abstract | The invention discloses a method for screening β-alanine synthase by double fluorescence method. A fluorescent reporter gene and a β-alanine synthase gene are co-introduced into host bacteria, inoculated with a basic salt medium containing arabinose, and After inducing and culturing at 30-37°C, a fermentation broth was obtained; the fermentation broth was frozen and thawed, and the disrupted solution was the crude enzyme solution; the crude enzyme solution was added with L-aspartic acid, and allowed to stand at 37°C for 2 hours Carry out the conversion reaction and convert the substrate into β-alanine, get the transformation solution and measure the fluorescence value under the conditions of absorption wavelength 355nm and emission wavelength 445nm; Get fermentation broth and measure fluorescence value under the condition of absorption wavelength 395nm and emission wavelength 509nm, The high-activity β-alanine synthase is obtained by screening; the system of the invention has the advantages of simple operation, stable enzyme activity measurement, and high enzyme activity detection sensitivity, and has important application value in the directional transformation of β-alanine synthase. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112662732-A |
| priorityDate | 2019-01-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 67.