http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109880827-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01K67-027 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-113 |
| filingDate | 2019-03-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-05-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-05-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109880827-B |
| titleOfInvention | Method for establishing hepatolenticular degeneration zebra fish model |
| abstract | The invention discloses a method for establishing a hepatolenticular degeneration zebra fish model, which comprises the following steps: designing a gRNA target sequence on an amino acid structural domain coded by exon 2 of a zebra fish ATP7B gene, and synthesizing a primer; preparing a gRNA in-vitro transcription template by using a pMD19-gata5_ gRNA scaffold vector as a template, and performing in-vitro transcription, purification and recovery to obtain a gRNA; the gRNA and the Cas9mRNA are injected into a zebra fish embryo at a single cell stage, and the ATP7B mutant zebra fish is obtained by culture and screening. |
| priorityDate | 2019-03-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 60.