http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109870433-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 |
| filingDate | 2017-12-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-06-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-06-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109870433-B |
| titleOfInvention | Buoyancy microsphere probe for quantitative screening of ganglioside and preparation method thereof |
| abstract | The invention relates to a pair of buoyancy microsphere probes for quantitatively screening cell surface ganglioside and a preparation method thereof. The buoyant microsphere probe comprises an extraction probe and a collection probe. The extraction probe takes a silica buoyancy microsphere modified by maleimide as a substrate, and is covalently bonded with a DNA molecule with an endonuclease cutting site and a boric acid terminal. Through covalent recognition of boric acid and sialic acid on the cell surface, the extraction probe can extract biomolecules containing sialic acid on the cell surface through buoyancy. The extracted biomolecules can be released into the solution under the action of endonuclease, and the ganglioside with the nucleic acid fragments combined therein is collected by a collection probe silanized by octadecane through hydrophobic effect, and further fluorescence quantification is carried out on the ganglioside by using in vitro hybridization chain reaction. |
| priorityDate | 2017-12-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 44.