http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109867727-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-866 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P31-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K19-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-39 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-235 |
| filingDate | 2019-02-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-06-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-06-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109867727-B |
| titleOfInvention | Flagellin-fiber2 fusion protein, and preparation method and application thereof |
| abstract | The invention provides a flagellin-fiber2 fusion protein, a preparation method and application thereof, wherein the fusion protein has fusion protein of avian adenovirus 4 fiber2 protein and salmonella typhimurium flagellin with high immunoprotection. The preparation method comprises the steps of fusing and cloning the artificially encoded flagellin-fiber2 gene into a pFastBacc-HA expression vector, transposing the gene to form recombinant Bacmid, then transfecting the recombinant Bacmid into Sf9 insect cells, expressing the fusion protein by using a baculovirus system, and identifying by indirect immunofluorescence IFA and Western blot. The period of obtaining the recombinant baculovirus by the system is short, and the flagellin-fiber2 fusion protein is used for immunizing SPF (specific pathogen free) chickens, so that the flagellin-fiber2 fusion protein expressed by the baculovirus system has high immune protection capability. |
| priorityDate | 2019-02-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 405.