http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109867721-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61Q19-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-78 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K8-65 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-34 |
| filingDate | 2019-04-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-11-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-11-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109867721-B |
| titleOfInvention | Recombinant stichopus japonicus collagen polypeptide, preparation method and application thereof |
| abstract | The invention provides a recombinant stichopus japonicus collagen polypeptide, wherein the nucleotide sequence of the encoded recombinant stichopus japonicus collagen polypeptide is SEQ ID NO: 2, the preparation method mainly comprises the following steps: s1, cloning genes; s2, constructing a vector plasmid: integrating a target gene sequence into a vector plasmid, and introducing the integrated plasmid into competent genetic engineering bacteria; s3, fermentation and thallus collection: selecting the positive genetic engineering bacteria clone obtained in the step S2, placing the positive genetic engineering bacteria clone in an LB liquid culture medium to culture to reach a target concentration, and then centrifugally collecting bacteria; s4, purification and dialysis: the bacteria collected in step S3 are subjected to protein purification after removing impurities, and then subjected to protein dialysis. The preparation method in the scheme can be used for efficiently preparing the stichopus japonicus collagen polypeptide, and solves the problems of high sea cucumber culture and capture cost, high impurity content, low purity and the like in the current application of the sea cucumber collagen polypeptide; simple production conditions and steps, easily controlled reaction conditions, low production cost and low purity. |
| priorityDate | 2019-04-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 64.