http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109837282-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-415 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-29 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-06 |
| filingDate | 2017-11-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-08-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-08-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109837282-B |
| titleOfInvention | Expression and purification of citrus allergen Cit s 1.01 antigen, preparation and application of polyclonal antibody |
| abstract | The invention discloses antigen expression and purification of citrus allergen Cit s 1.01, preparation and application of polyclonal antibody, and specifically discloses a codon-optimized Rutaceae plant allergen Cit s 1.01 gene, which is characterized by comprising SEQ ID NO: 2 shows the sequence. In the present invention, the prokaryotic expression sequence of the Cit s 1.01 gene is optimized, the gene is synthesized, the prokaryotic expression system of the allergen Cit s 1.01 is constructed, and the expressed and purified rCit s 1.01 protein is used to prepare the rabbit polyclonal antibody Anti-Cit s 1.01. The allergen Cit s 1.01 content in the pulp was detected, and the potential allergenicity of the allergen Cit s 1.01, such as the edible part of citrus fruits, could be evaluated by measuring the strength of Western blot by optical density value. |
| priorityDate | 2017-11-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 225.