http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109837280-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9c8c98a38f38e8f0ca609b2b6540a82e |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02A40-80 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-79 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-113 |
| filingDate | 2019-03-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_827021cff3bc809fa782008e5bc443b2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_47bfd6e1c50d50c5f5866f5f147bbd18 |
| publicationDate | 2019-06-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109837280-A |
| titleOfInvention | A kind of seaweed endogenous temperature-inducible promoter and its application |
| abstract | The invention belongs to the technical field of seaweed genetic engineering, and relates to an endogenous temperature-inducible promoter of seaweed and its application. The promoter contains coexisting high temperature heat shock element HSE and low temperature cold shock element LTR. The present invention adopts technologies such as chromosome walking, gene cloning, DNA sequencing and the like to obtain the heat shock protein 70 gene (hsp70) promoter of the large seaweed Enteromorpha, which has the sequence shown in SEQ ID NO: 1; and further identified through deletion mutation and genetic transformation. The cis-elements responsible for high and low temperature response in the promoter and their interaction mechanism; on this basis, the cloned heat shock protein 70 gene promoter was inserted into the upstream of the reporter gene GUS, and the transformation vector was constructed and introduced into the thallus of E. prolifera. Temperature-induced expression of the source gene GUS gene. The technology can improve the controllability and biosafety of the transgenic prolifera, and is of great significance to the improvement of algal species and the development of genetically engineered products. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-115804340-A |
| priorityDate | 2019-03-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 153.