http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109825611-B
Outgoing Links
Predicate | Object |
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classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6888 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6804 |
filingDate | 2019-04-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2020-11-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2020-11-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-109825611-B |
titleOfInvention | Method and kit for rapidly detecting goose-derived components in food |
abstract | The invention provides a rapid detection kit for goose-origin components in food, and relates to the technical field of biological species identification. The invention firstly screens out a goose-source universal internal standard gene LOC106029425, the nucleotide sequence of which is shown as SEQ ID NO.1, the copy number of which is constant in goose species, no allelic variation exists, and the goose-source universal internal standard gene can be used as a target gene for identifying goose sources. An LAMP amplification primer is designed by taking the gene as a target sequence, and is subjected to constant-temperature amplification together with LAMP reaction liquid, and an LAMP reaction product is used for detecting the colloidal gold nucleic acid test strip. The LAMP reaction is combined with the colloidal gold nucleic acid test strip for detection to form a rapid detection kit, so that the goose-source components in food can be rapidly and sensitively detected, and the detection sensitivity can reach 0.16% (w/w). The kit has the advantages of simple use method, low cost, easy observation of reaction results and good specificity, and is very suitable for field real-time detection. |
priorityDate | 2019-04-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 60.