http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109825531-B
Outgoing Links
Predicate | Object |
---|---|
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-07 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-75 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 |
filingDate | 2019-01-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2021-07-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2021-07-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-109825531-B |
titleOfInvention | Method for removing pXO2 plasmid in Bacillus anthracis |
abstract | The invention discloses a method for removing pXO2 plasmid in Bacillus anthracis. The method for removing the pXO2 plasmid in the bacillus anthracis is completed by adopting a CRISPR/Cas9 system, the CRISPR/Cas9 system comprises sgRNA with the name of sgRNA2, a target sequence recognized by the sgRNA2 is a specific sequence contained in pXO2 plasmid and not contained in the bacillus anthracis, and the target sequence is a DNA fragment shown in the 1 st to 20 th sites of the sequence 4 in a sequence table. The method adopts the CRISPR/Cas9 system to remove the bacillus anthracis virulence large plasmid pXO2 plasmid, successfully obtains the bacillus anthracis without pXO2 plasmid, has simple operation, provides a faster, more convenient and new means for constructing a new vaccine strain, and provides a new thought for the prevention and the treatment of the bacillus anthracis. |
priorityDate | 2019-01-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 491.