| abstract |
The invention discloses a preparation method and application of a novel Mycobacterium tuberculosis fusion strain. The regeneration conditions of BCG strain protoplasts and H37Ra strain protoplasts are optimized, and regenerated strains with good activity and growth are cultivated. A method for preparing a fusant of BCG strain protoplast and H37Ra strain protoplast is provided, which utilizes electroporation technology to fuse the two protoplasts, explores the optimal fusion conditions, and simultaneously regenerates the fusant to obtain growth. Good regenerating strain. The present invention uses BCG and H37Ra strains as parental strains and uses protoplast fusion technology to successfully construct a new type of tuberculosis vaccine fusion strain, which has excellent biological characteristics and genetic traits of BCG and H37Ra strains, and explores the immune protection efficacy and safety of this fusion strain. , to provide a basis for further exploration and research on the possibility of this fusion strain becoming a candidate tuberculosis vaccine. |