http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109825465-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-125 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-75 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 |
| filingDate | 2019-02-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-11-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-11-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109825465-B |
| titleOfInvention | Recombinant bacillus subtilis for synthesizing lactyl-N-neotetraose based on balanced UDP-sugar supply and construction method and application thereof |
| abstract | The invention discloses a method for synthesizing lactyl-N-neotetraose based on balanced UDP-sugar supply, wherein recombinant Bacillus subtilis is obtained by culturing Bacillus subtilis 168 delta amyE: P in a host bacterium 43 ‑lacY，P 43 ‑lgtB，P xylA -comK having two beta-1, 4-galactosyltransferases integrated on the genome and integrating glucose-6-phosphate isomerase gene, glutamine-fructose-6-phosphoaminotransferase gene, UTP-glucose-1-uridine phosphateAcyl transferase gene, UDP-glucose-epimerase gene, and knocking out UDP-glucose 6-dehydrogenase gene, and exogenously expressing beta-1, 3-N-glucosyl aminotransferase gene. The recombinant bacillus subtilis constructed by the invention synthesizes the lactoyl-N-neotetraose, the yield on a shake flask can reach 1800mg/L, and the efficient synthesis of the lactoyl-N-neotetraose is realized. |
| priorityDate | 2019-02-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 1367.