http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109811042-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6858 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 |
| filingDate | 2018-12-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-02-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-02-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109811042-B |
| titleOfInvention | Method for detecting folic acid metabolism related gene mutation by combining overlap extension PCR with Sanger sequencing |
| abstract | The invention discloses a method for detecting folate metabolism related gene mutation by combining overlapping extension PCR with Sanger sequencing, which is characterized in that a primer combination for detecting the folate metabolism related gene mutation is provided, the nucleotide sequence of the primer combination is shown as SEQ ID No. 1-8, the detection method established by the invention is established by using the primer combination, the single nucleotide polymorphism of the folate metabolism related gene locus is detected by combining the overlapping extension PCR with Sanger sequencing, only one PCR reaction and one sequencing reaction are needed in the whole process, the number of the PCR reactions and the number of the sequencing reactions are greatly reduced, the 6 mutation loci of MTHFR and MTRR genes related to folate metabolism can be detected, and new variation loci in amplified fragments can be found by using the method. The detection technology has important clinical significance when being applied to the detection of the single nucleotide polymorphism of the folic acid metabolism related gene locus, not only simplifies the operation steps and reduces the detection cost, but also improves the detection efficiency, and is worthy of great popularization. |
| priorityDate | 2018-12-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 31.