http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109810996-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d1a291fb43e0fc4fbd3e1a262d96750d http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_55e2eaacd3e6e29155cfebf0b7af9e3d |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-01 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63 |
| filingDate | 2019-01-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4656d92f6981a087968949b5d65efd8d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_326d54360017e2c289c478a92eaa10bf http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_198a84be9ab6c8977392a6c7af26e585 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_78c41b64d8b31bc543428c86ae9ce1e7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7dd19d5fe80fea4fa35ff09a94e58892 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_084bcf2fdb54fd9f6e5313204a42f9da |
| publicationDate | 2019-05-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109810996-A |
| titleOfInvention | A kind of cloning vector carrying Chinese yellow mosaic virus and its construction method and application |
| abstract | The invention belongs to the technical field of genetic engineering, in particular to a cloning vector carrying the Chinese yellow mosaic virus and a construction method and application thereof. The cloning vector carrying the Chinese yellow mosaic virus includes Agrobacterium containing 1.5 times the full-length recombinant subsequence of DNA A and Agrobacterium containing 2 times the full-length recombinant subsequence of DNA β. After resuspension of Agrobacterium GV3101-A and Agrobacterium GV3101-β, they were mixed at a volume ratio of 1:1 to obtain a mixed bacterial solution, and then the mixed bacterial solution was injected into the tobacco seedlings with a 1 mL sterile syringe, 2-3 weeks after the inoculation Tobacco plants carrying the Chinese yellow mosaic virus can be obtained. The infectious clone of the present application can infect N. benthamiana and N. benthamiana with high efficiency, which lays a material foundation for studying the pathogenicity of the virus and screening of disease-resistant tobacco germplasm resources. |
| priorityDate | 2019-01-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 31.