http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109810958-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-865 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P11-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-81 |
| filingDate | 2019-03-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-10-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-10-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109810958-B |
| titleOfInvention | Saffron-derived CCD2 mutant, coding sequence and application thereof, and recombinant yeast strain for producing crocetin |
| abstract | The invention relates to the technical field of genetic engineering, and discloses a saffron-derived CCD2 mutant, a coding sequence and application thereof, and a recombinant yeast strain for producing crocetin. The CCD2 mutant provided by the invention has one or more than two of mutations at V120F, Y190K/A, R192V/F, E211A, E212A, T290V, K320A and S323F/A/T sites. The invention is modified aiming at the rate-limiting enzyme CCD2 in the synthesis path of the crocetin, realizes the direct synthesis of the crocetin from the beta-carotene by widening the substrate spectrum of the CCD2, simplifies and reconstructs the existing synthesis path of the crocetin, thereby solving the problem of generally low total yield of multi-step reaction; meanwhile, the efficiency of synthesizing the crocetin by catalyzing the original substrate zeaxanthin by the CCD2 is improved, and a yeast strain with high yield of the crocetin is obtained. |
| priorityDate | 2019-03-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 42.