http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109810157-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02P20-55 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H15-26 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H17-075 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C09K11-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H1-00 |
| filingDate | 2018-12-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-05-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-05-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109810157-B |
| titleOfInvention | A kind of β-glucuronidase precipitation type fluorescent substrate synthesis method |
| abstract | The invention discloses a method for synthesizing a β-glucuronidase precipitation type fluorescent substrate based on 2-(benzothiazole-2'-yl)-4-bromophenol. The synthesis method includes the following three-step reactions: 1) glycosylation reaction; 2) aromatic cyclization reaction; 3) deprotection group reaction. In the synthesis method, the reaction yield of each step can be above medium, and the highest is more than 90%, the reaction conversion rate of materials with relatively high price or preparation cost is relatively high, the total yield of three steps can reach 37%, and the reaction conditions are mild, Easy to implement. At the same time, the glycosylation reaction step and the deprotection group reaction step in the synthesis method can be applied to synthesizing a common fluorescent substrate 4-methylumbelliferone-β-D using 4-methylumbelliferone as a glycosyl acceptor -Glucuronide, the two-step reaction yields can reach 51% and 89% respectively, and the total yield can reach about 45%. |
| priorityDate | 2018-12-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 59.