abstract |
A method for detecting residual amount of Pichia host protein in recombinant human lysozyme raw material, comprising preparing recombinant human lysozyme, preparing Pichia host protein, detecting the total protein content of Pichia host protein, and preparing Pichia host protein Antiserum, elimination of cross-reaction between recombinant human lysozyme and Pichia host protein immunized rabbit serum and Pichia host protein immunized sheep serum, two-dimensional electrophoresis of Pichia host protein, detection of antibody coverage of Pichia host protein, enzyme Combined immunoassay steps. The detection method of the invention is used to detect the Pichia pastoris host protein in the recombinant human lysozyme in three consecutive batches. The detection method of the invention has the advantages of strong specificity, complete antibody coverage, no cross-reaction and the like, and can be used for the content detection of Pichia pastoris host protein. |