http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109797165-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-74 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-81 |
| filingDate | 2018-07-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-07-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-07-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109797165-B |
| titleOfInvention | Method for improving yield of dibasic acid by traceless editing technology |
| abstract | The invention relates to a method for improving the yield of dibasic acid by a traceless editing technology. The application of the CRISPR-Cas 9-based Candida tropicalis genome traceless editing vector in improving the yield of dibasic acid is disclosed, and the nucleotide sequence of the CRISPR-Cas 9-based Candida tropicalis genome traceless editing vector is shown in SEQ ID No. 1. The vector constructed by the invention comprises Cas9, sgRNA, homology arms, a forward screening marker and a replication initiation site of prokaryotes. Through detection, the recombinant strain prepared by the invention can greatly improve the yield of the dibasic acid, lays a foundation for industrial production of the dibasic acid, and provides an application reference for traceless editing of microbial genes such as other yeasts, corynebacterium glutamicum, escherichia coli and the like. |
| priorityDate | 2018-07-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 71.