| abstract |
The present invention provides a kind of digital pcr chips and preparation method thereof, preparation facilities, application method, digital pcr chip includes: the structure sheaf (12) of substrate (11) and setting above substrate (11), structure sheaf (12) has at least one sample channel, each sample channel includes the microcellular structure with multiple micropores (121) and the spill cavity (122) for holding extra sample, and spill cavity (122) is located on at least side of microcellular structure.The case where technical solution of the present invention prevents extra sample from overflowing, it is easy to use, detection efficiency is improved, low manufacture cost, yield are high, improve sample throughput, improve PCR reaction heat conduction efficiency. |