| abstract |
Reported herein is a method for determining the introduction of a nucleic acid into the genome of a mammalian cell, wherein the mammalian cell comprises one or both transcriptionally active alleles of the DPH1, DPH2, DPH4 and/or DPH5 genes, the method comprising Steps: Transfect mammalian cells with one or more plasmids containing the nucleic acid to be introduced and the elements required for gene editing of the DPH gene, and culture the transfected cells in the presence of DPH gene transcription-sensitive toxins. cells, and if the transfected cells survive in the presence of the toxin, the nucleic acid is determined to be introduced into the genome of the mammalian cell. |