http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109777808-B
Outgoing Links
Predicate | Object |
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classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-29 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-65 |
filingDate | 2018-12-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2020-09-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2020-09-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-109777808-B |
titleOfInvention | Polypeptide and application thereof in positioning expression of foreign protein in cell nucleus and chloroplast |
abstract | The invention relates to a polypeptide and application thereof in positioning expression of exogenous protein in cell nucleus and chloroplast, belongs to the field of molecular genetics, and provides a DNA sequence for encoding the polypeptide, application thereof in positioning expression of exogenous protein in cell nucleus and chloroplast and a related method. In particular to a sequence from a moso bamboo genome, a polypeptide coded by the sequence can express exogenous protein in chloroplast and nucleus, can be used as a marker gene for co-localization of chloroplast and nucleus, and provides a reliable comparison method for subcellular localization. The invention firstly clones a sequence of a phyllostachys pubescens gene, and introduces yellow fluorescent protein connected with the C end into chloroplast and cell nucleus. The transit peptide is fused with the fluorescent protein, observation in living cells can be carried out, time and labor are saved, and the method is a basic method for researching the functions of the protein. |
priorityDate | 2018-12-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 108.