http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109777771-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0775 |
| filingDate | 2019-03-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-08-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-08-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109777771-B |
| titleOfInvention | Serum-free culture medium for primary umbilical cord mesenchymal stem cells and use method thereof |
| abstract | The invention relates to a serum-free culture medium of primary umbilical cord mesenchymal stem cells and a use method thereof, wherein the serum-free culture medium of the primary umbilical cord mesenchymal stem cells comprises the following components: alpha-MEM culture, AMD3100, BMP-4, insulin, FGF-2, IL-6, and LIF. Fetal calf serum is not added into the culture medium, so that immunological rejection possibly generated in clinical application is avoided, and the risk of pathogenic microorganism pollution is reduced; by adding cytokines such as IL-3, IL-6, G-CSF, SCF and the like, the strong proliferation effect can be generated by the cooperation with FL, and the differentiation of umbilical cord mesenchymal stem cells can be inhibited; the EGF is added to cooperate with the FGF-2 to promote the proliferation and differentiation of the umbilical cord mesenchymal stem cells; by adding BMP-4 as a nutritional factor for the proliferation and differentiation of stem cells, the normal proliferation state of umbilical cord mesenchymal stem cells can be maintained and regulated. |
| priorityDate | 2019-03-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 257.