http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109762809-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate | 2019-03-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-06-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-06-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109762809-B |
| titleOfInvention | Method for extracting cfDNA in sputum specimen |
| abstract | The invention relates to the field of DNA extraction methods, and provides a method for extracting cfDNA from a sputum specimen, which comprises the steps of preparing an extracted sample of cfDNA in the sputum and extracting the cfDNA from the extracted sample by using a cfDNA extraction kit, wherein the preparation method of the extracted sample of cfDNA in the sputum comprises the following steps: 1) collecting sputum; 2) controlling the quality of the sputum sample; 3) mixing the sputum specimen with the phlegm-resolving liquid, and shaking until the phlegm is completely resolved to obtain a phlegm-resolving mixed liquid; 4) centrifuging the phlegm-reducing mixed solution, and separating a supernatant and a bottom precipitate, wherein the supernatant is a cell-free supernatant; 5) and centrifuging the cell-free supernatant, and collecting the supernatant to obtain an extraction sample. The method for extracting cfDNA from the sputum specimen provides a standardized extraction process of cfDNA from the sputum specimen, and can obtain sufficient cfDNA from the sputum specimen to provide a basis for subsequent gene change detection. |
| priorityDate | 2019-03-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 55.