http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109762808-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate | 2019-03-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-06-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-06-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109762808-B |
| titleOfInvention | Phlegm-resolving liquid for extracting free DNA in cell-free supernatant of sputum specimen |
| abstract | The invention provides a phlegm-resolving liquid for extracting free DNA in cell-free supernatant of a sputum specimen, which consists of normal saline and dithiothreitol aqueous solution with the concentration of 0.1-0.7 mol/L; the normal saline and the dithiothreitol water solution are independently packaged and stored before use; mixing the normal saline with the sputum specimen and the dithiothreitol aqueous solution at the use site; the volume ratio of the physiological saline to the dithiothreitol aqueous solution is 9: 1. The formula reagent is easy to obtain, the preparation method is simple, and the wide popularization is facilitated; compared with the sputum reducing reagent applied to clinic, the reagent has advantages in the aspects of sputum reducing effect, the amount of obtained cfDNA and the accuracy of gene change detection result; because the obtained cfDNA is abundant, the method can be used for detecting various genes; the formula is isotonic liquid, after cell-free supernatant is collected in a short-term treatment, cell morphology cannot be damaged by adding cell fixing liquid into a precipitate, and a subsequent cytological pathological diagnosis result is not influenced. |
| priorityDate | 2019-03-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 76.