abstract |
The invention discloses a D-amino acid mixture medium and a preparation method and application thereof. Adding the D-amino acid mixture to the medium can improve the acid resistance of Lactococcus lactis and the production of nisin (Nisin). The original strain was fermented in the medium with D-amino acid mixture for 12h, and the Nisin production reached the maximum at 8h, the original strain was increased by 1.5-1.7 times, and the 8-h acid stress survival rate was increased by 1.4-1.7 times. The D-amino acid mixture of the present invention has a significant impact on the product Nisin and the stress resistance of the strain, wherein Nisin is a safe and non-toxic food preservative, and the D-amino acid is widely used in food, medicine and the like. |