http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109750063-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H6-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H5-00 |
| filingDate | 2019-03-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-04-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-04-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109750063-B |
| titleOfInvention | CRISPR _ Cas9 vector construction of histone deacetylase gene HDA19 and application thereof |
| abstract | The invention relates to the field of kenaf molecular breeding, in particular to construction and application of a CRISPR _ Cas9 vector of histone deacetylase gene HDA 19. The vector is a T1-sgRNA expression cassette, a T2-sgRNA expression cassette and a T3-sgRNA expression cassette which insert three target points of kenaf HDA19 gene on a vector pYLCRISPR-Cas9 Pubi-H. According to the principle of designing targets by the CRISPR _ Cas9 technology, the invention designs three targets on three exons of the kenaf HDA19 gene, successfully constructs a pYLCRISPR-Cas9Pubi-H-HDA19 vector, and provides a basis for researching the effect of the HDA19 gene in kenaf male sterility generation and regulation and control mechanisms of salt, drought and other adversity stresses. The invention provides a theoretical basis for the research on the molecular mechanism of regulating the generation of the male sterility of the kenaf by histone acetylation, and provides feasibility for creating a male sterility material by utilizing an engineering technology in the future. |
| priorityDate | 2019-03-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 40.