http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109744149-B
Outgoing Links
Predicate | Object |
---|---|
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H4-00 |
filingDate | 2019-03-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2022-05-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2022-05-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-109744149-B |
titleOfInvention | Method for tissue culture and propagation of children vegetables by using bud blocks |
abstract | The invention belongs to the technical field of baby vegetable planting, and discloses a method for tissue culture and propagation of baby vegetables by using bud blocks, which comprises the steps of cutting excellent plant axillary buds by using a scalpel, and filling the excellent plant axillary buds into a nylon mesh bag; cutting axillary buds into a plurality of small axillary bud blocks, and inoculating the small axillary bud blocks on a pre-culture medium 1/2 MS; inoculating in a solution containing 0.1 mg.L ‑1 NAA and 0.3 mg.L ‑1 6-BA induction culture medium; adding 0.3 mg.L to MS proliferation medium with adventitious bud after 30 days of culture as explant ‑1 6-BA and 0.1 mg. L ‑1 And (4) selecting strong seedlings to be inoculated into a rooting culture medium after NAA is subjected to subculture for 30 d. The invention selects single plants with excellent target characters as materials to carry out tissue culture and rapid propagation of the sedge, the test-tube plantlet is transplanted to a field after overwintering without vegetative growth and directly enters reproductive growth so as to obtain good-quality seeds, and the selective breeding of the good seeds of the sedge is carried out, thereby providing high-quality seeds for production. |
priorityDate | 2019-03-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 20.