| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_653c418e41edbb4e1ce8bc5e53e70cec |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-327
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6825
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-48
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-543
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-569
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-571 |
| filingDate |
2019-01-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1cefb29a7a5d37056163f2b906a9dbc1
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cf33d807aa69c1fa43c2275ab81a5ef5
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b695fea8934d2380d13de1002f910672
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_99ab80e196b5bd97089ebb73458fd24d |
| publicationDate |
2019-05-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CN-109738503-A |
| titleOfInvention |
A Positive Feedback Amplified Electrochemical Sensor Based on Exonuclease III |
| abstract |
The invention discloses a positive feedback amplification electrochemical sensor based on exonuclease III, comprising a gold electrode and a Hybrid DNA fixed on the gold electrode, wherein the Hybrid DNA sequentially includes sulfur-hydrogen bonds from the 5' end to the 3' end , a double-stranded DNA structure and a single-stranded DNA structure, the double-stranded DNA structure is prepared by hybridizing C-DNA with G4-DNA and A-DNA, and the base sequence of the single-stranded DNA structure is the same as that of the T-DNA. The base sequences are complementary; Hybrid DNA is immobilized on the gold electrode via S‑Au bonds. A positive-feedback amplification strategy combining homogeneous and heterogeneous reactions is achieved through cleverly designed Hairpin DNA and Hybrid DNA and Exo III-based target cyclic amplification. This strategy exhibits ultra-high sensitivity with a detection limit as low as 0.12aM T-DNA and high selectivity for T-DNA and other base mismatch nucleic acids. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111004836-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111004836-B
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110146566-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110501411-B
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110501411-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112410330-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111500686-A |
| priorityDate |
2019-01-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |