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filingDate 2019-02-20-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2019-08-06-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2019-08-06-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-109738500-B
titleOfInvention The preparation method of nano-complex and its label-free aptamer electrochemistry gamma interferon sensor
abstract The invention discloses a kind of preparation methods based on the label-free aptamer electrochemistry gamma interferon sensor of dendrimer/Jenner's grain of rice/molybdenum disulfide nano compound.The drop coating compound prepares modified electrode in electrode surface, and gamma interferon aptamer chain terminal sulfydryl is connect by Au-S key with Jenner's grain of rice, and the modified electrode of compound-aptamer is made.In the presence of gamma interferon, aptamer chain is specifically bound on gamma interferon and sensor, is caused aptamer hairpin structure to be opened stretching, extension, can effectively be adsorbed the methylene blue MB in electrolyte, MB redox signal is caused to significantly increase.The linear relationship being fitted between MB oxidation peak current intensity and gamma interferon concentration, constructs label-free aptamer electrochemistry gamma interferon sensor.Compared with prior art, the method for the present invention is easy to operate, low in cost, high sensitivity, selectivity are good, can develop into a kind of novel label-free aptamer electrochemical sensor, the highly sensitive and highly selective detection for gamma interferon in biological sample.
priorityDate 2019-02-20-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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