Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7641d8ec4b289baa8268f4936e0e4635 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-861 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P31-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N7-01 |
filingDate |
2019-01-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2ea52bde6f7948751afaa6e949731f8f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_59299f130f89a221ba57a300cf80a594 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3ca9f605761752eeb61ab28e1eaef3a0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6563a92bb5ee612ede97062716aa4bdd http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1fe46e4913e8cf3fc27e4c8a048eaf5d |
publicationDate |
2019-05-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
CN-109735567-A |
titleOfInvention |
Construction of a recombinant adenovirus vector co-expressing African swine fever EP153R and P54 genes and adenovirus packaging method |
abstract |
The invention provides a recombinant adenovirus vector construction and an adenovirus packaging method in which African swine fever EP153R and P54 genes are co-expressed, belonging to the technical field of genetic engineering. An EP153R gene and P54 gene over-expression adenovirus vector is based on the pShuttle-CMV eukaryotic expression vector, and CTLA4, EP153R and P54 genes are introduced into the multi-cloning site. The present invention also provides a recombinant adenovirus co-expressing EP153R and P54 genes, using the constructed pAD-Shuttle-CMV-CTLA4-EP153R-P54 vector to realize the adenovirus packaging process to obtain an adenovirus that can directly infect animals or eukaryotic cells , thereby realizing the purpose of co-expression of EP153R and P54 genes in eukaryotic cells, and laying a foundation for the study of adenovirus vaccines with co-expression of EP153R and P54 genes. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2022007800-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113913462-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114377121-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112522286-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112592937-A |
priorityDate |
2019-01-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |