abstract |
A kind of double-antibodies sandwich ELISA of quick detection infecting both domestic animals and human pathogen Aeromonas, belongs to technical field of immunoassay.The present invention is through screening cross reaction, the sensitivity of different pairing antibody, and using 14H5 as coated antibody, 1B1-HRP has cross reaction to the bacterium in the Aeromonas of test as the sandwich ELISA method that enzyme labelled antibody is established.The present invention is prepared for the monoclonal antibody of Aeromonas specificity, and establishes Pseudomonas specific diabodies Sandwich ELISA, provides accurate, reliable, quick analysis means for the detection of aquatic pathogenic bacterium Aeromonas. |