http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109734745-B
Outgoing Links
Predicate | Object |
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classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-44 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-77 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-795 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-543 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-107 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-765 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07F9-24 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07F9-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-79 |
filingDate | 2018-12-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2021-05-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2021-05-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-109734745-B |
titleOfInvention | Preparation method and application of fenitrothion antibody |
abstract | The invention discloses a preparation method and application of an antibody for directly identifying fenitrothion. The structural formula of the hapten of fenitrothion is shown as a formula (II) or (I): ; . And then coupling the hapten to carrier protein to successfully prepare two artificial antigens. Preparing a hybridoma cell capable of secreting an anti-fenitrothion antibody by immunizing an animal with one of the artificial antigens; one of the artificial antigens is used as an envelope antigen, and a fenitrothion detection method based on an indirect competitive enzyme linked immunosorbent assay is further established. The detection method for detecting fenitrothion pesticide residue has the characteristics of simplicity, convenience, rapidness, strong specificity and higher sensitivity, and the detected IC 50 The value was 7.13ng/mL, and the detection limit was (IC) 10 )1.7ng/mL, and the linear detection range is 2.9-17.9 ng/mL. |
priorityDate | 2018-12-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 55.