http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109725162-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_47756d499764082e32fd76e2bf383210 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-535 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-533 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-74 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-76 |
| filingDate | 2018-12-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0b4d6c5ee783ceef39f195f92920d0ce http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1680f9c78fe27a8c5ee4a35e52c625a3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_effe766250b582c740bafe040c9c2e48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5dd45fc6af624490b801685029f7aa7f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a5a2730d4b478eea352dd360ace4fc68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1d3254e922a830089d9b28415dc888f2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1dd172ab4a271491668eae2b06ce4e3f |
| publicationDate | 2019-05-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109725162-A |
| titleOfInvention | A detection kit and method for completely homogeneous determination of insulin |
| abstract | The invention belongs to the field of biotechnology and discloses a detection kit and a method for completely homogeneous determination of insulin. The invention is based on double antibody sandwich technology, acridinium ester-labeled anti-human insulin antibody and horseradish peroxidase-labeled anti-human insulin antibody are respectively combined on the antigenic determinant of insulin molecule to form an antibody-antigen-antibody immune complex, After adding the triggering agent, the horseradish peroxidase oxidation triggering agent produces hydroxyl radicals, and the half-life of hydroxyl radicals is very short, and under the action of antioxidants, it can ensure that hydroxyl radicals are in the immune complex of antibody-antigen-antibody. Intramolecular transmission, acridinium ester generates light signal under the action of hydroxyl radical, and its light signal is positively correlated with insulin content. The kit of the invention can realize complete homogeneous chemiluminescence detection of insulin, does not need immobilized antibodies, and does not need a washing process, and has the characteristics of good stability, fast and convenient detection, high sensitivity and strong specificity. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112881675-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111521830-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112904003-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113125421-A |
| priorityDate | 2018-12-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 183.