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filingDate 2018-12-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e82dd0b16c6b736c387b0b62f14ea6be
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publicationDate 2019-05-07-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-109724956-A
titleOfInvention Detection method of lepidopteran microparticle protozoan spores, dyeing solution and preparation method thereof
abstract The invention discloses a lepidopteran insect microparticle protozoa spore dyeing solution, which is a phosphate buffer solution containing isothiocyanate. Accordingly, the inventors have also established a preparation method of the staining solution and a detection method using the staining solution. The detection method adopts the aforementioned staining solution to fluorescently dye the sample, and then observe the spores under a fluorescence microscope. The invention is applied to fluorescently dye the sporozoites of lepidopteran insect protozoa, and the production is simple and easy to operate; dyeing with a fluorescent microscope and lepidopteran insect protozoan spore staining solution makes the insect protozoan spores have fluorescence specificity; control The shape characteristics of the standards ensure repeatability and accuracy of the test. In a word, the invention has the characteristics of simple operation, strong specificity and easy identification, lays a foundation for mechanical inspection and fair inspection, and has high practical value and broad market prospect.
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