http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109722716-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0cbd749eb61f56e76c45e6b8351d8062 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B50-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-13 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63 |
| filingDate | 2019-01-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_966f6d56c5fe7a4bfcb15ee03664bf7d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e968759283a3423ff29449922a08ec92 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_19e7d4c3e26efa4c27968ce9c06c6ec8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e67f980960a2ba7474418a6cec07b421 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9d45738f3e336fdb144e3076747ee743 |
| publicationDate | 2019-05-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109722716-A |
| titleOfInvention | A method for constructing a melanoma nanobody library |
| abstract | The invention provides a method for constructing a melanoma nanobody library, which belongs to the technical field of bioengineering. The melanoma cells are cultured and then crushed, and 200ug/time of subcutaneously immunized alpaca four times; the sheep are collected one week after the fourth immunization. The camel anticoagulated blood, separated lymphocytes, extracted total RNA, and reverse transcribed into cDNA; using cDNA as the template, PCR amplification was performed with the first pair of primers, and the 700bp amplification product was recovered; using the 700bp amplification product as the template, the first The VHH fragments were amplified by PCR with two pairs of primers, and then ligated with pCANTAB5e after being digested twice with SfiI respectively; the obtained ligation products were electrotransformed into TG1 competent cells to obtain a melanoma nanobody library. The melanoma nanobody library constructed by the construction method of the present invention is an immune library with good characteristics and is suitable for specifically screening nanobodies related to melanoma growth, development, migration, proliferation and the like. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114671949-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114671949-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113150140-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111269318-A |
| priorityDate | 2019-01-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 646.