http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109722481-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_653c418e41edbb4e1ce8bc5e53e70cec |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6825 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6886 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-327 |
| filingDate | 2018-12-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ab4bca6ae5a6e320b086c5e4b0a66ddd http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e062285cecc1687a86dfbfc07e73a34e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a616f0763ff34c4808bd0cffcce02a42 |
| publicationDate | 2019-05-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109722481-A |
| titleOfInvention | Detection of microRNAs in lung cancer cells based on a substrate-free and label-free electrocatalytic amplification biosensor |
| abstract | The invention discloses a method for detecting microRNA in lung cancer cells based on a substrate-free, non-labeled electrocatalytic amplification biosensor. The biosensor is prepared by heating dicyandiamide and ferrous salt to 490 ℃ in an inert gas atmosphere. The precursor is formed by self-assembly at ~510 °C, the precursor is heated to 890 ~ 910 °C in an inert gas atmosphere, and iron-containing nitrogen-rich carbon nanotubes are obtained after calcination; the iron-containing nitrogen-rich carbon nanotubes and gold nanoparticles are modified to glassy An AuNP/FeCN/GCE electrode was obtained from the carbon electrode, and then a thiolated capture probe was immobilized on the AuNP/FeCN/GCE electrode through a gold-sulfur bond, and the capture probe was a single-stranded DNA capable of hybridizing with the target microRNA. In the present invention, in the absence of H 2 O 2 , a large amount of introduced thionine is electrocatalyzed and reduced, and the electrochemical signal is greatly enhanced. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110687171-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110927238-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110687171-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111982993-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111982993-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110927238-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113533467-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113533483-A |
| priorityDate | 2018-12-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 145.