http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109706204-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-38 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N11-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N11-084 |
| filingDate | 2019-01-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-05-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-05-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109706204-B |
| titleOfInvention | A method for preparing thymidine from immobilized Escherichia coli |
| abstract | The invention belongs to the field of biocatalysis, in particular to a method for preparing thymidine by immobilizing Escherichia coli. In the present invention, PVA and carrageenan are used as embedding substrates, and activated carbon is used as additives to immobilize Escherichia coli producing phosphorylase to obtain immobilized Escherichia coli; in the reaction for preparing thymidine, immobilized Escherichia coli is used as a catalyst, and Phosphate buffer is used as solvent, 2-deoxy-α-D-ribose-1-phosphoric acid dicyclohexylamine salt and thymine are reaction substrates to produce thymidine. The invention adopts immobilized Escherichia coli to prepare thymidine, which better improves the tolerance of the bacteria to the substrate, improves its stability, and improves the yield of preparing thymidine; the results show that after purification, the thymidine The purity can reach more than 97%, and the yield can reach more than 90%; after 6 batches of reactions, the immobilized Escherichia coli has no obvious loss of activity. |
| priorityDate | 2019-01-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 148.