http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109706200-B

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classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-12
filingDate 2017-10-26-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2021-03-05-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2021-03-05-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-109706200-B
titleOfInvention Method for preparing laminaribiose
abstract The invention discloses a method for preparing laminaribiose by constructing an in-vitro multienzyme molecular machine and through multienzyme cascade catalysis, belonging to the field of enzymatic preparation of laminaribiose. The preparation method of the laminaribiose disclosed by the invention comprises the steps of converting glucose units in starch into glucose-1-phosphate by glucan phosphorylase, and synthesizing the laminaribiose by the laminaribiose phosphorylase through the glucose-1-phosphate and the glucose. The utilization rate of starch and the final concentration of laminaribiose can be further improved by adding other auxiliary enzymes such as isoamylase and glucanotransferase which promote complete phosphate-lysis of starch during the process. The method has the advantages of cheap and easily available substrate, low production cost, high product yield, simple separation and purification, and the like, and can realize the large-scale production of laminaribiose.
priorityDate 2017-10-26-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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