http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109706181-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-91 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-867 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-071 |
| filingDate | 2019-01-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-06-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-06-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109706181-B |
| titleOfInvention | Method for constructing immortalized porcine hepatic stellate cell line, immortalized porcine hepatic stellate cell line and application |
| abstract | The invention provides a method for constructing an immortalized porcine hepatic stellate cell line, the immortalized porcine hepatic stellate cell line and application, and relates to the technical field of immortalized cells. The porcine hepatic stellate cells are immortalized by adopting the lentiviral vector containing the SV40LT sequence, and the porcine hepatic stellate cells have the advantages of capability of infecting non-dividing-stage cells, large capacity of containing exogenous gene segments, capability of expressing for a long time and the like. The immortalized porcine hepatic stellate cell line constructed by the method grows in a stretching state, has pseudopodia, clear cell outline and complete cells, and has typical morphological characteristics similar to those of primary porcine hepatic stellate cells; after the obtained immortalized porcine hepatic stellate cell line is passaged for 10 generations, the cell morphology and the characteristics have no obvious difference with the cells of the 1 st generation, and the constructed immortalized porcine hepatic stellate cell line can be applied to drug screening. |
| priorityDate | 2019-01-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 86.