http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109705174-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H15-234 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H1-00 |
| filingDate | 2019-02-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-09-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-09-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109705174-B |
| titleOfInvention | Extraction method of tobramycin |
| abstract | The invention provides a method for extracting tobramycin, comprising: (1) adjusting the pH of 5'carbamoyl tobramycin fermentation filtrate to 7.0-7.5, adding cationic resin for adsorption, eluting with water and ammonia water, and collecting the ammonia water eluent; (2) concentrating the eluent; (3) hydrolyzing the obtained concentrated solution under alkaline conditions, adjusting the pH of the hydrolysis solution to 5.0-6.0, decolorizing with a cationic resin, collecting the decolorizing solution, and adjusting the pH to 7.0 ~7.5, pass through the self-prepared column of medium pressure chromatography equipped with cation resin, elute with water and ammonia water, collect the ammonia water eluent, then decolorize by strongly basic anion resin, and collect the decolorization solution; (4) the obtained decolorization solution is concentrated After decolorization with activated carbon, and then spray drying, the finished product of tobramycin is obtained. The method simplifies the production process, uses ultra-nanofiltration combined with medium-pressure chromatography self-prepared column technology, shortens the production cycle, has no solvent crystallization, no residual solvent, and reduces environmental protection discharge and processing pressure. |
| priorityDate | 2019-02-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 24.