http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109696546-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-56916 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-54326 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-569 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-543 |
| filingDate | 2019-02-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-04-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-04-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109696546-B |
| titleOfInvention | Method for detecting yersinia enterocolitica |
| abstract | The inventionDisclosed is a method for detecting yersinia enterocolitica, comprising the steps of: (1) taking a sample to be tested under the aseptic condition, adding Al (OH) into the sample 3 Homogenizing in PBS buffer solution to obtain sample bacterial liquid; (2) carrying out water-bath heat treatment on the sample bacterial liquid, centrifuging, discarding the supernatant, and collecting thalli; (3) adding yersinia enterocolitica immunomagnetic beads into the thalli, oscillating, standing and layering on a magnetic frame, and discarding the upper layer to obtain the yersinia enterocolitica immunomagnetic beads adsorbed with the thalli; eluting yersinia enterocolitica immunomagnetic beads adsorbed with thalli by using eluent, then adding PBS buffer solution into the eluted yersinia enterocolitica immunomagnetic beads, and uniformly mixing to obtain a sample solution to be detected; (4) and culturing, observing and detecting the sample solution to be detected. The detection method provided by the invention has strong specificity and high sensitivity, and can effectively shorten the detection time and reduce the detection cost. |
| priorityDate | 2019-02-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 42.