http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109680026-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2770-32323 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02A50-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2770-32334 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K2039-5258 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K2039-55505 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K39-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K39-39 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P31-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-005 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-78 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-125 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-085 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P31-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-39 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-34 |
| filingDate | 2019-02-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-12-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-12-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109680026-B |
| titleOfInvention | Purification of recombinant CA16 virus-like particles, application in vaccines and vaccines |
| abstract | The invention provides a purification method of the recombinant CA16 virus-like particle, its application in vaccines and the vaccine. Through the high-density fermentation culture of recombinantly expressed engineering bacteria and methanol-induced expression of CA16 virus particle protein, the bacteria were collected by centrifugation and subjected to high-pressure homogenization and crushing, and the supernatant was subjected to ultrafiltration, ion exchange chromatography, and hydroxyapatite chromatography. It can be obtained after purification by molecular sieve chromatography. The CA16 class virus particle vaccine provided by the present invention has good immunogenicity, safety, immune characteristics and biological activity, simple process, and chromatography method is adopted for purification. Compared with density gradient centrifugation, it is more conducive to linear amplification, and can be used on a large scale It is prepared and purified, and a high-purity (more than 99%) VLP stock solution can be obtained, which can be used to prepare a vaccine for preventing CA16 infection, and has good economic value and application prospects. |
| priorityDate | 2019-02-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 158.