http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109679993-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8205 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H4-001 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H4-008 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H4-00 |
| filingDate | 2019-02-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-11-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-11-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109679993-B |
| titleOfInvention | Construction method of agrobacterium rhizogenes-mediated transgenic plant |
| abstract | The invention provides a method for constructing transgenic plants mediated by agrobacterium rhizogenes, which relates to the technical field of plant genetic engineering, and the method comprises the steps of firstly constructing agrobacterium rhizogenes with target genes, enabling aseptic seedlings of plants to root and then planting the aseptic seedlings, and injecting a bacterial liquid of the agrobacterium rhizogenes with the target genes into stems of the aseptic seedlings of the plants when the stem length of the planted aseptic seedlings exceeds 3cm from the roots; and after transgenic hairy roots of the plants grow out from the stem injection part, culturing until the length of the transgenic hairy roots exceeds 3cm and the number of the roots is more than 10, removing the non-hairy roots, and stretching the hairy roots into soil to obtain the hairy root transgenic plants. Compared with the conventional plant transgenic system, the method can save the step of redifferentiation of the plant callus cells and overcome the problems of long period and the like existing in the genetic transformation by utilizing the callus cells. The method of the invention has the advantages of simple required equipment, easy mastering of operation technology and wide development and application prospect. |
| priorityDate | 2019-02-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 61.