http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109679986-B
Outgoing Links
Predicate | Object |
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classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-6437 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-82 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y304-21021 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-64 |
filingDate | 2017-10-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2022-08-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2022-08-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-109679986-B |
titleOfInvention | Application of Plants as Hosts in the Expression of Seven Coagulation Factors |
abstract | The present invention relates to the field of biotechnology, in particular to the application of plants as hosts in expressing the seven coagulation factors. The present invention utilizes recombinant vector and Agrobacterium-mediated vacuum infiltration method to express human blood coagulation seven factors (anti-hemophilic factor (AHF), FVII, FVIIa, proconvertin). The expression system confirms that the plant exogenous protein can be collected after 4 days of Agrobacterium infection. The successful expression of recombinant FVII was confirmed by SDS-PAGE and Western blotting (Western method). The blood coagulation experiment proved that the FVII produced by lettuce has biological activity. The present invention provides a method for low-cost, convenient and mass production of active recombinant human FVII. |
priorityDate | 2017-10-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 317.