http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109666627-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2506-1369 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-05 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-115 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-135 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-31 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-999 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-30 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0683 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-071 |
| filingDate | 2019-02-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2023-01-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2023-01-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109666627-B |
| titleOfInvention | Method for inducing umbilical cord mesenchymal stem cells to differentiate into Leydig cells with small molecules |
| abstract | The present invention provides a method for inducing the differentiation of human umbilical cord mesenchymal stem cells into Leydig cells by small molecules, which includes the following steps: ① Obtain the human umbilical cord mesenchymal stem cells from human umbilical cords, and culture the human umbilical cord mesenchymal stem cells using a differentiation medium. Human umbilical cord mesenchymal stem cells. ②Add an early differentiation inducer to the differentiation medium to induce early differentiation. ③Add proliferation inducer to the differentiation medium for early induction of proliferation. ④ Add a metaphase differentiation inducer to the differentiation medium to induce metaphase differentiation. ⑤ adding the proliferation-inducing agent to the differentiation medium to induce proliferation in the middle stage. ⑥Add a mature differentiation inducer to the differentiation medium to induce late mature differentiation. ⑦ Manual removal of non-testis Leydig cells. ⑧ After removing the Leydig cells, the remaining cells were continued to be cultured with the enriched medium, and finally the target Leydig cells were obtained. |
| priorityDate | 2019-02-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 186.