http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109655435-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2021-6432 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6486 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6428 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 |
| filingDate | 2018-11-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-08-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-08-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109655435-B |
| titleOfInvention | Fluorescence quenching test paper for detecting okadaic acid and preparation method and application thereof |
| abstract | The invention belongs to the technical field of immunoassay, and particularly relates to a fluorescence quenching test paper for detecting okadaic acid, and a preparation method and application thereof. The fluorescence quenching test paper comprises a sample pad, a combination pad, a nitrocellulose membrane, a water absorption pad and a PVC base plate, wherein the combination pad is sprayed with an OA monoclonal antibody marked by colloidal gold particles, a detection line on the nitrocellulose membrane is a mixed solution of fluorescent microspheres and OA-BSA, and a quality control line is the fluorescent microspheres. The fluorescence quenching test paper has strong specificity, no cross reaction, 1.56ppb of sensitivity, 9.6 times of improvement compared with the colloidal gold test paper with the same parameters, 3.12-50 ppb of detection limit and 9min of detection time. The obtained fluorescence quenching test paper has high stability on interference of the shellfish tissue, can quantify OA toxin by matching with a simple fluorescence immunochromatographic instrument and data analysis software, is intuitive in result judgment, is not easy to cause misjudgment of non-professional personnel, and has huge development and application prospects. |
| priorityDate | 2018-11-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 125.