| abstract |
The invention relates to a method for preparing mature red blood cells in vitro by using peripheral blood and a preparation, in particular to a method for preparing mature red blood cells in vitro by using Lin in peripheral blood ‑ CD34 ‑ Method for preparing mature red blood cells by cells, comprising enriching Lin ‑ CD34 ‑ Cells, differentiation Lin into erythroid cells ‑ CD34 ‑ Cells and promoting the enucleation maturation of erythrocytes; the mature red blood cells prepared by the method, a differentiation medium and a cytokine combination applied in the mature red blood cells are also provided, and the preparation method has the following advantages: the raw materials are easy to obtain, the yield of the red blood cells is high, and the obtained red blood cells have good performance. |