http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109629009-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B50-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B50-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6869 |
| filingDate | 2019-01-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-02-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-02-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109629009-B |
| titleOfInvention | RAD-seq-based noninvasive PGS (somatic mutation in somatic cell culture) method for embryos |
| abstract | The invention discloses a method for performing noninvasive PGS on embryos based on RAD-seq. The invention provides a method for preparing a DNA library, which comprises the following steps: constructing a DNA library by taking a blastocyst culture solution as a sample; two restriction enzymes were used for disruption of genomic DNA during library construction. The invention also provides a method for RAD-seq of the embryo, which comprises the following steps: (1) constructing a DNA library by taking a blastocyst culture solution as a sample; in the process of constructing the library, two restriction endonucleases are adopted to break the genome DNA; (2) sequencing the DNA library constructed in the step (1). The invention adopts the blastula culture solution in the operation of the test-tube baby as the raw material to analyze the chromosome aneuploidy condition of the embryo, thereby evaluating the development potential of the embryo and providing a new method for selecting the correct embryo in the assisted reproduction. |
| priorityDate | 2019-01-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 101.