http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109628397-B
Outgoing Links
Predicate | Object |
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classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-84 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-2315 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-2318 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-2302 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-599 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0646 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0783 |
filingDate | 2019-02-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2023-01-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2023-01-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-109628397-B |
titleOfInvention | A kind of NK cell in vitro expansion culture method |
abstract | The invention relates to a method for culturing and amplifying human NK cells in vitro, comprising: coating a culture bottle with heparin sodium, CD16 and human immunoglobulin, collecting peripheral blood, inactivating plasma, separating mononuclear cells, using sterile Serum medium (containing autologous plasma, IL‑2, IL‑15 and IL‑18) to culture and expand cells, cell harvesting and detection and other steps. The invention does not use trophoblast cells or magnetic beads to sort and purify, and has simple operation, high safety, good amplification effect and high clinical application value. |
priorityDate | 2019-02-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 19.